Fig. 4
CGRP and amylin are expressed in different vesicles and fibres in the DRG. Antibodies: anti-amylin (mAb254259), anti-CGRP (pAb36001) and anti-CTR (rat: mAb8B9; human: mAb31-01/1H-10). Examples of (A) rat and (B) human DRG neurons which express both amylin and CGRP. Arrows indicate examples of vesicles which display immunoreactivity for one peptide but not the other. Filled white arrowheads indicate examples of positive immunoreactivity; empty arrowheads indicate examples of an absence of immunoreactivity. C Examples of rat DRG fibres that are immunoreactive for one peptide but not the other. D Examples of peptide immunoreactivity in human DRG fibres. Examples of (E) rat and (F) human DRG CTR immunoreactivity relative to neuronal fibres (β tubulin III). Filled white arrowheads indicate examples of positive staining; empty arrowheads indicate examples of an absence of staining. Image brightness and contrast were adjusted for presentation purposes and merged in FIJI. Scale bar, 10 μm. Images are representative of (A) three (one male, two female) or (C, E) six (three male, three female) rats, or (B, D, F) four human cases (two male, two female). In human DRG, CTR could not be visualised in the same section due to equipment restraints/limitations and secondary antibody combinations