Fig. 3

CTR, CGRP and amylin are expressed in DRG neurons. Immunofluorescent localisation of CTR, CGRP and amylin in (A) rat or (B) human DRG using anti-amylin (mAb254259), anti-CGRP (pAb36001) and anti-CTR (rat: mAb8B9; human: mAb31-01/1H-10). Filled arrowheads indicate examples of positive immunoreactivity; empty arrowheads indicate examples of an absence of immunoreactivity. Magenta arrowheads indicate inset location. Image brightness and contrast were adjusted for presentation purposes and merged in FIJI. Scale bar, 100 μm. Images are representative of six rats (three male, three female) or four human cases (two male, two female). C The percentage of CTR-positive DRG neurons that also co-express amylin, CGRP, or amylin and CGRP together. CTR only neurons refers to the population of CTR-positive neurons which do not express amylin or CGRP. D The distribution of size of neurons co-expressing CTR, CGRP and amylin together was quantified relative to the number of neurons which co-express CTR/CGRP/amylin for rat and human DRG