Fig. 2

CTR is expressed in DRG neurons. Immunofluorescent localisation of CTR with β tubulin III or NF200 in (A) mouse, B rat, and (C) human DRG using anti-CTR (mouse/rat: pAb188/10; human: mAb31-01/1H-10) antibodies. Filled arrowheads indicate examples of positive immunoreactivity; empty arrowheads indicate examples of an absence of immunoreactivity. Yellow arrowheads indicate inset location. Image brightness and contrast were adjusted for presentation purposes and merged in FIJI. Scale bar, 100 μm. Images are representative of six mice or rats (three male, three female) and four human cases (two male, two female). D The percentage of total DRG neuronal population (β tubulin III-positive cells) which express CTR. Negative neurons refers to the population of neurons (β tubulin III-positive cells) which do not express CTR. E The distribution of neuron size quantified relative to the total β tubulin III expressing neuron population. F cAMP production in response to rat αCGRP and amylin in neuron-enriched neonatal rat DRG cultures. Data points are mean ± s.e.m, combined from 7–9 independent experiments performed in duplicate or triplicate