Fig. 1

Amylin and CGRP are expressed in DRG neurons. Immunofluorescent localisation of amylin and CGRP with β tubulin III or NF200 for (A) mouse, (B) rat and (C) human DRG using anti-amylin (mAb254259) and anti-CGRP (pAb36001) antibodies. Filled arrowheads indicate examples of positive immunoreactivity; empty arrowheads indicate examples of an absence of immunoreactivity. Magenta arrowheads indicate inset location. Image brightness and contrast were adjusted for presentation purposes and merged in FIJI. Scale bar, 100 μm. Images are representative of six mice or rats (three male, three female) and four human cases (two male, two female). D Validation of the rat Iapp RNA-FISH probe in transfected HEK293S cells, images representative of three independent experiments. E RNA-FISH detection of rat Iapp mRNA in the presence or absence of 1.6 µM rat Iapp probe in rat C1 DRG. Fluorescent in situ hybridization shown in greyscale and nuclear staining in blue. Scale bar, (D, E) 100 μm or (E, insets) 50 μm. Images are representative of results from three individual rats (two male, one female). F The distribution of neuron size quantified relative to the total β tubulin III expressing neuron population and the percentage of total rat and human DRG neuronal population (β tubulin III) which express amylin alone, CGRP alone, or co-express amylin and CGRP together (immunohistochemistry only). Negative neurons refers to the population of neurons (β tubulin III-positive cells) which do not express amylin or CGRP. Size distribution of the mouse DRG neurons could not be performed due to limitations with the image analysis