Fig. 2

Establishment and evaluation of recurrent NTG-induced CM mouse model. a Basal mechanical thresholds in the periorbital region and hindpaw plantar area were assessed in the CM and control (VEH) groups (n = 9). b Representative photos of calcitonin gene-related peptide (CGRP) immunoreactive staining in the trigeminal nucleus caudalis (TNC) of each group (scale bar = 200 μm) and mean CGRP fluorescence intensity were compared (n = 6). c Representative western blotting (WB) results for CGRP in each group (n = 5). All data were presented as mean ± SEM (standard error of mean). Behavioral data were assessed by two-way repeated-measures ANOVA with post hoc comparison between groups. IF and WB data were assessed by the independent sample t-test or Mann–Whitney U test. (*P < 0.05, **P < 0.01, ***P < 0.001)