Fig. 6

Anxa10-203 over-expression enhanced Mc1r mRNA stability through DHX30. a, b RNA and protein expression of DHX30 in Sham and CCI mice with or without DHX30 silenced. *P < 0.05, **P < 0.01, ***P<0.001, n = 4/group. c, d Expression of Mc1r in Sham and CCI-ION mice with or without DHX30 silenced was assessed by RT-qPCR (c) and WB (d). *P < 0.05, **P < 0.01, n = 3/group for RT-qPCR, n=4/group for WB. e, f The expression of DHX30 mRNA (e) and protein (f) were evaluated in the TGNs with DHX30 knockdown and Anxa10-203 over-expression in vitro. *P < 0.05, **P < 0.01, n = 4/group. g, h The expression of Mc1r mRNA (g) and protein (h) was evaluated in TGNs with DHX30 silenced and Anxa10-203 over-expressed in vitro. *P < 0.05, **P < 0.01, ***P < 0.001, n = 4/group for RT-qPCR, n=3/group for WB. i Representative images of nascent protein in TGNs of the two groups. Scare bar = 20 μm. j Quantitative analysis of fluorescence intensity of nascent protein in the two groups. n = 10/group. k The decay of Mc1r mRNA in different groups. *P < 0.05, ***P < 0.001, n = 4/group. oe: over-expression, n.s: no significance