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Fig. 3 | The Journal of Headache and Pain

Fig. 3

From: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

Fig. 3

DHX30 protein interacted with Anxa10-203 RNA. a Venn diagram of the proteins from the RNA pull-down-LCMS and RNA-protein interaction online algorithm (CatRAPID and RBPsuite). b Gene Ontology analysis showed the top 10 enriched biological process, cellular component, and molecular function of these overlapped proteins. c RNA-FISH and IF staining revealed the co-localization of DHX30 protein and Anxa10-203 RNA in the cytoplasm of TGNs. Scare bar = 50 μm. d RNA pull-down assay assessed Anxa10-203 RNA interacted with DHX30 protein, the antisense served as control. **P < 0.01, n = 3/group. e RIP assay further confirmed that DHX30 protein interacted with Anxa10-203 RNA in the TG of CCI-ION mice. *P < 0.05, n = 3/group. f The scores of Anxa10-203-DHX30 binding sites predicted by RBPsuite. g The Anxa10-203 sequence segments that interacted with DHX30 protein were evaluated by RNA pull-down

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