Fig. 5

Chemogenetic modulation of noradrenergic neuron activity in the LC significantly influences the impact of sleep deprivation on pain perception (VEH, n = 5 mice; CNO, n = 5 mice; ASD, n = 5 mice; CNO+ASD, n = 5 mice). A Schematic representation of the timeline elucidating the modification of the mechanical pain response induced by ASD and subsequent intervention employing chemogenetic inhibition and activation. B and C Temporal dynamics of changes and statistical trends in the periorbital mechanical threshold (B) and right hind paw threshold (C) induced by chemogenetic activation. D and E Temporal dynamics of changes and statistical trends in the periorbital mechanical threshold (D) and right hind paw threshold (E) induced by chemogenetic inhibition. Data are expressed as the mean ± SEM. Two-way ANOVA with Tukey's post hoc test for multiple comparisons. * P < 0.05; ** P < 0.01; ***P < 0.001. (B: 26h: VEH vs. CNO+ASD P = 0.005. There was no significant difference in the comparative weight of other groups at baseline, 24h and 26h. C: VEH vs. CNO P = 0.012, vs. ASD P = 0.244, vs. CNO+ASD P < 0.001; CNO vs. ASD P= 0.546, vs. CNO+ASD P < 0.001; ASD vs. CNO+ASD P < 0.001. There was no significant difference in the comparative weight of other groups at baseline and 24h. D: VEH vs. CNO P = 0.017, vs. ASD P = 0.938, vs. CNO+ASD P = 0.991; CNO vs. ASD P= 0.003, vs. CNO+ASD P = 0.008; ASD vs. CNO+ASD P = 0.991. There was no significant difference in the comparative weight of other groups at baseline and 24h. E: 26h: CNO vs. ASD P=0.008. There was no significant difference in the comparative weight of other groups at baseline, 24h and 26h)