Fig. 3

Administration of α2 receptor agonists/antagonists through microinjection in the LC can modify pain perception and sleep architecture. A Schematic representation of the timeline demonstrating the change in mechanical pain triggered by agonists/antagonists, as measured via von Frey tests (n = 6 mice). B/C Temporal dynamics of changes and statistical trends in the periorbital mechanical threshold (B) and right hind paw threshold (C) induced by agonists/antagonists. D Schematic representation of the timeline demonstrating the change in sleep architecture triggered by agonists/antagonists (n = 6 mice). E/F Statistical trends in sleep latency (E) and sleep time (F) induced by agonists/antagonists. Data are expressed as the mean ± SEM. Two-way ANOVA with Tukey's post hoc test for multiple comparisons and one-way repeated-measures ANOVA for multiple comparisons. * P < 0.05; ** P < 0.01; ***P < 0.001. (B: Base CLON vs. VEH P = 0.998, vs. YO P = 0.778, VEH vs. YO P = 0.825; 1h: Base CLON vs. VEH P < 0.001, vs. YO P < 0.001, VEH vs. YO P = 0.090; 2h: Base CLON vs. VEH P = 0.002, vs. YO P < 0.001, VEH vs. YO P = 0.009.C: Base CLON vs. VEH P = 0.631, vs. YO P = 0.998, VEH vs. YO P = 0.598; 1h: Base CLON vs. VEH P = 0.024, vs. YO P < 0.001, VEH vs. YO P = 0.021; 2h: Base CLON vs. VEH P = 0.852, vs. YO P < 0.001, VEH vs. YO P = 0.003.E: Base vs. CLON P = 0.049, vs. YO P = 0.029; CLON vs. YO P = 0.006. F: Base vs. CLON P = 0.035, vs. YO P = 0.045; CLON vs. YO P = 0.003)