Table 1 Antibody used in western blotting and immunofluorescence staining
Antibody | Manufacturer | Catalog number | Host | Dilution |
|---|---|---|---|---|
For western blot | ||||
TREM1 | Abcam, UK | ab217161 | Rabbit | 1:1000 |
c-fos | Santa Cruz, USA | sc166940 | Mouse | 1:500 |
CGRP | Abcam, UK | ab139264 | Rabbit | 1:1000 |
p-p65 | CST, USA | 3033 | Rabbit | 1:1000 |
p-65 | CST, USA | 8242 | Rabbit | 1:1000 |
p-IκBα | CST, USA | 2859 | Rabbit | 1:1000 |
IκBα | CST, USA | 4814 | Mouse | 1:1000 |
NLRP3 | CST, USA | 15101 | Rabbit | 1:1000 |
ASC | CST, USA | 67824 | Rabbit | 1:1000 |
Cleaved-Caspase-1 | Proteintech, China | 22915-1-AP | Rabbit | 1:2000 |
Mature IL-1β | Abcam, UK | ab234437 | Rabbit | 1:1000 |
Mature IL-18 | Abcam, UK | ab191860 | Rabbit | 1:2000 |
β-actin | Santa Cruz, USA | sc-47778 | Mouse | 1:1000 |
HRP conjugated anti-rabbit | Proteintech, China | SA00001-2 | Goat | 1:100000 |
HRP conjugated anti-mouse | Proteintech, China | SA00001-1 | Goat | 1:100000 |
For immunofluorescence | ||||
TREM1 | Abcam, UK | ab217161 | Rabbit | 1:200 |
NLRP3 | Proteintech, China | 68102-1-Ig | Mouse | 1:100 |
CGRP | Santa Cruz, USA | sc-57053 | Mouse | 1:100 |
Iba1 | Abcam, UK | ab5076 | Goat | 1:500 |
GFAP | Santa Cruz, USA | sc-33673 | Mouse | 1;200 |
NeuN | Abcam, UK | ab104224 | Mouse | 1:1000 |
Alexa Fluor 488 Donkey anti-mouse IgG | Jackson ImmunoResearch, USA | 715-545-150 | Donkey | 1:200 |
Alexa Fluor 594 Donkey anti-mouse IgG | Jackson ImmunoResearch, USA | 715-585-150 | Donkey | 1:200 |
Alexa Fluor 488 Donkey anti-rabbit IgG | Jackson ImmunoResearch, USA | 711-545-152 | Donkey | 1:500 |
Alexa Fluor 594 Donkey anti-goat IgG | Jackson ImmunoResearch, USA | 705-585-147 | Donkey | 1:500 |