Fig. 7

NF-κB activity modulates microglial activation and inflammatory response after NTG administration. a-f Immunoblot analysis of NLRP3, cleaved-caspase-1, ASC, mature IL-1β, and mature IL-18 after administration of NTG and PDTC (n = 6). g Immunofluorescence images of Iba1 in the TNC of mice treated with NTG and PDTC. Scale bar: 20 μm. h The magnified image of Iba-1 corresponds to that within the white dotted line frame. i–l Immunofluorescence quantification of Iba-1-positive microglia, immunoreactivity, and total and mean initial neurite length per microglia (n = 5). Three slices were taken from each mouse for analysis, with each slice containing 4-6 FOVs. Values are the mean ± SEM; one-way ANOVA and Tukey's post hoc tests; ***p < 0.001 vs. the VEH group and ^###p < 0.001 vs. the NTG group