Fig. 4

TREM1 is critical for the activation of TNC microglia and inflammatory response after NTG administration. a-f Immunoblot analysis was conducted to detect the expression levels of NLRP3, cleaved-caspase-1, ASC, mature IL-1β, and mature IL-18 after the administration of NTG and LR12 (n = 6). g Immunofluorescence images of Iba1 in the TNC of mice treated with NTG and LR12. h The magnified image of Iba-1 corresponds to that within the white dotted line frame. i–l Immunofluorescence quantification was performed to measure Iba-1-positive microglia, Iba1 immunoreactivity, total neurite length per microglia, and mean initial neurite length per microglia (n = 5). Three slices were taken from each mouse for analysis, with each slice containing 4-6 FOVs. Scale bar: 20 μm. The values are the mean ± SEM; one-way ANOVA and Tukey's post hoc tests; **p < 0.01 and ***p < 0.001 vs. the VEH group; ^##p < 0.01, ^###p < 0.001 vs. the NTG group; ^&&p < 0.01 vs. the VEH-LR12 group