Fig. 5

Propranolol dampens LC functioning. A Simultaneous recordings from the TCC and the LC were made from naïve rats; after baseline measures, they received either IS (meninges) + aCSF (LC) or IS (meninges) + propranolol (LC), and measures were repeated after 30min. B Ratio of LC early component (in percentage of response above BL normalized by BL) to TCC A response (total number of spikes for the train) evoked by cutaneous electrical stimulation after IS and then propranolol or aCSF microinjection in the same animals (n = 7). C Index of synchronisation among spontaneous LC MUA activity (normalized power in delta frequency) before and after IS and propranolol or aCSF microinjection (n = 8). D-E Examples of firing patterns (current-clamp mode) evoked by depolarizing (D) or ramp currents (E) before, during propranolol application and after washout. F Variation in rheobase before and after bath-applied propranolol (n = 4). Symbols in the middle of graphs show values before and after propranolol for each individually recorded neuron. Horizontal line in boxplots represents median, bottom and top edges 25th and 75th percentiles respectively, and dots represent individual values. * P < 0.05, ** P < 0.01; paired Student’s t test for (B) and (C)