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Fig. 3 | The Journal of Headache and Pain

Fig. 3

From: Progesterone distribution in the trigeminal system and its role to modulate sensory neurotransmission: influence of sex

Fig. 3

Double immunohistochemistry of progesterone and CGRP or RAMP1. A Progesterone and CGRP: Progesterone (red) and CGRP (green) immunoreactive neurons (arrows) and in fibers (arrowheads) and their co-localization in the merged picture showed that there was no co-localization between them. B Progesterone and RAMP1: Double staining of progesterone and RAMP1 showed that in some neurons progesterone (red) and RAMP1 (green) expressed in same type of neurons but in different location (arrow in the merged image). Progesterone was most prominently expressed in the assumed cell membrane with some immunoreactivity within the cytoplasm, while RAMP1 was mainly localized in the cell cytoplasm

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