Fig. 6

ShRNA-BMP7 induced OL apoptosis in VC. A Immunofluorescence of APC (in pink) and TUNEL (in red) in the VC of the shRNA-GFP and shRNA-BMP7 groups at day 21 after the AAV injection. White arrows indicate cells with TUNEL-positive APC staining and DAPI (in blue) labels nucleated cells; scale bar = 50 μm. B Quantification of cells with TUNEL-positive APC staining from (A); n = 3 per group. C, D Western blotting and the corresponding quantification for Bax and Bcl2 in the VC of CON, shRNA-GFP, and shRNA-BMP7 groups at day 21 after the AAV injection; n = 3 per group. Compared with the shRNA-GFP group, **p < 0.01, ****p < 0.0001. shRNA = short hairpin RNA; TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; VC = trigeminal spinal subnucleus caudalis; AAV = adeno-associated virus