Fig. 4

ShRNA-BMP7 induced mechanical allodynia and demyelination. A MWT of rats in the shRNA-GFP and shRNA-BMP7 groups at the corresponding time points after the AAV injection; n = 6 per group. B–D mRNA and protein levels of BMP7 in the VC of control group (CON), shRNA-GFP group and shRNA-BMP7 group at day 21 after AAV injection; n = 3 per group. E–I mRNA and protein levels of OL markers (CNPase and MBP) in the VC of CON, shRNA-GFP, and shRNA-BMP7 groups at day 21 after AAV injection; n = 3 per group. mRNA levels in (B), (E), and (F) are presented as a fold change from the CON group mRNA level. Protein bands depicted in (C) and (G) have the corresponding optical densities labeled in kiloDaltons (kD). Quantifications of these bands are shown in (D), (H), and (I), normalized by the GAPDH loading control. Compared with the shRNA-GFP, *p < 0.05, **p < 0.01, ****p < 0.0001. Compared with the baseline (P0), ^#p < 0.05, ^####p < 0.0001. shRNA = short hairpin RNA; VC = trigeminal spinal subnucleus caudalis; AAV = adeno-associated virus