Fig. 2

ION-CCI induced mechanical allodynia and demyelination with a reduction of BMP7. A MWT of rats in the CCI group and the Sham group at the indicated time points post-operation (P); n = 6 per group. B TEM images showed the myelin profiles in the VC of Sham and CCI groups at day 21 after ION-CCI; scale bar = 20 μm. C G-ratios of myelin with different diameters that were calculated from (B); n = 3 per group. D mRNA levels measured by qPCR of BMP7 and CNPase in the VC of CCI and Sham groups at the corresponding time points post-operation (P); n = 3 per group. mRNA quantification is presented as a fold change of CCI/Sham. E, F Protein levels measured by western blotting of BMP7 and CNPase in the VC of CCI and Sham groups at the corresponding time points post-operation; n = 3 per group. The bands are imaged in (E) and the quantification is presented in (F). GAPDH is used as the loading control. The optical densities of each band are indicated in kiloDaltons (kD). Compared with the Sham group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Compared with the baseline (P0), ^####p < 0.0001. MWT = Mechanical withdrawal threshold; ION-CCI = Infraorbital-nerve chronic constriction injury; VC = left trigeminal spinal subnucleus caudalis; TEM = Transmission electron microscopy