Fig. 10

AAV-BMP7 activated STAT3 and p65. A–C Protein levels of phosphorylated (p)-STAT3, STAT3, p-p65, and p65 in the VC of each group at day 21 after the AAV injection; n = 3 per group. Compared with the CCI + AAV-GFP group, ^###p < 0.001, ^####p < 0.0001. Compared with the Sham group, *p < 0.05. D The double immunoflurorescent staining of APC (in red) and P-STAT3 (in pink) in the VC of CCI + AAV-GFP and CCI + AAV-BMP7 groups at day 21 after the AAV injection. White arrows indicate P-STAT3-positive APC staining and DAPI (in blue) labels nucleated cells; scale bar = 20 μm. E Quantification of cells with P-STAT3-positive APC staining from (D); n = 3 per group. F The double immunoflurorescent staining of APC (in red) and P-P65 (in pink) in the VC of CCI + AAV-GFP and CCI + AAV-BMP7 groups at day 21 after the AAV injection. White arrows indicate P-P65-positive APC staining and DAPI (in blue) labels nucleated cells; scale bar = 20 μm. G Quantification of cells with P-P65-positive APC staining from (F); n = 3 per group. Compared with the CCI + AAV-GFP group, **p < 0.01. AAV = adeno-associated virus; VC = trigeminal spinal subnucleus caudalis; CCI = chronic constriction injury