Fig. 8

Pretreatment with 2R, 6R-HNK blockes TRPA1- or TRPV1- activated calcium influx in DRG neurons. a Statistical donut charts showed the percentage of responding neurons to allyl-isothiocyanate (AITC, an agonist of TRPA1 receptor) in different groups. b Representative images of Ca²⁺ responses in cultured DRG neurons before or during perfusion of AITC (100 μM, 120 s) and KCL (144 mM, 30 s) with HHBS or different dosage of 2R, 6R-HNK (3, 10, 30 μM) preincubation 3 h before trials. c Summary of Ca²⁺ responses in small (< 18 μm), medium (≥ 18 μm and ≤ 25 μm) and large (> 25 μm) DRG neurons pretreated with 2R, 6R-HNK or not. d-f Representative images (e), percentage of responding neurons (d) and time course plots (f) showing the changes in Ca²⁺ responses to capsaicin (Cap, TRPV1 agonist, 1 μM, 120 s) and KCL with or without 2R, 6R-HNK pretreatment, ^***p < 0.001, ^****p < 0.0001 compared with HHBS group