Fig. 4
PKCβ1 is involved in the adiponectin-mediated IT response. A Protein abundance of phosphorylated CaMKII (p-CaMKII) or total CaMKII (t-CaMKII) in TG cells treated with 100 nM adiponectin. Representative blots of at least 3 independent experiments are shown. B, Time course of IT changes (left) and bar chart (right) revealing the effect of adiponectin at 100 nM on IT in cells preincubated with 1 μM KT-5720 (n = 10 cells). Insets indicate representative current traces. Numbers represent points used for exemplary traces. C, Bar graph revealing that pretreatment of cells with 1 μM KT-5720 abrogated the 20 µM forskolin-induced IT response (n = 6 cells). ***p < 0.001 (vs. control), paired t test. D, Bar graph indicating that pretreatment of TG cells with CK2α-siRNA prevented the 100 nM adiponectin-induced increase in PKC activity. Data are means ± SEM from four independent experiments. **p < 0.01 (vs. vehicle), #p < 0.05 (vs. NC-siRNA + APN), unpaired t test. E–F, Time course of IT changes mediated by 100 nM adiponectin in cells pretreated with GF109203X (1 µM, D) or bisindolylmaleimide V (Bis-V, 1 µM, E). Insets indicate representative current traces. Numbers represent points used for exemplary traces. G, Bar graph revealing the effect of 100 nM adiponectin on IT in cells pretreated with GF109203X (n = 9 cells) or Bis-V (n = 8 cells). **p < 0.01 (vs. control), paired t test. H Bar graph revealing the effect of 100 nM adiponectin on IT in the presence of Go6976 (200 nM, n = 7 cells). ***p < 0.001 (vs. control), paired t test