Fig. 2
AdipoR1 mediates the adiponectin-induced increase in IT. A, B Protein abundance of adipoR1 (A) and adipoR2 (B) in the brain and TGs of intact mice. GAPDH was used as an equal loading control. Representative blots of at least 3 independent experiments are shown. C, Colocalization of adipoR1 (red) with NeuN, GS, NF200, CGRP, IB4 or Cav3.2 (green) in TG sections. Arrows show the colocalization. Scale bar, 50 µm. D, Protein abundance of adipoR1 in TG cells treated with control siRNA (NC-siRNA) or adipoR1 siRNA (adipoR1-siRNA). Representative blots of at least 3 independent experiments are shown. **p < 0.01 (vs. NC-siRNA), unpaired t test. E, Representative traces (left) and bar chart (right) revealing that adipoR1-siRNA treatment prevented the adiponectin-induced IT increase (n = 11 cells). Adiponectin at 100 nM significantly enhanced IT in cells transduced with NC-siRNA (n = 12 cells). **p < 0.01 (vs. control + NC-siRNA), unpaired t test