Fig. 1
Adiponectin enhances IT in small-sized TG neurons. A Representative traces (left) and bar chart (right) indicating the effect of nitrendipine (5 μM, n = 10 cells) or TTA-P2 (3 µM, n = 10 cells) on IT. Insets indicate the stimulation waveform. ***p < 0.001 (vs. pre-TTA-P2), paired t test. B Time course of IT changes induced by 100 nM adiponectin (APN). Insets indicate representative current traces. Numbers represent points used for exemplary traces. C Bar chart showing the enhancement of IT induced by 100 nM adiponectin (n = 10 cells). **p < 0.01 (vs. control), paired t test. D Dose‒response curves of adiponectin on IT. The solid line represents the best fit with the Hill equation. In brackets is the number of cells recorded at each concentration of adiponectin. E Current–voltage curve indicating the enhancement of 100 nM adiponectin on IT current density (n = 11 cells). IT was recorded from the holding potential of -110 mV with depolarizing pulses ranging from -80 to + 40 mV in 10-mV increments. *p < 0.05 (vs. control), one-way ANOVA. F Adiponectin at 100 nM did not alter the voltage-dependent activation properties of IT (n = 11 cells) but shifted steady-state inactivation properties in a depolarizing direction (n = 12 cells). Insets indicate stimulation protocols