Fig. 6
Inhibition of Panx1 channels prevents the CSD-related acute (at 30 min) elevation of MGS scores, oculotemporal (OT) (head grooming) strokes, and cortical nuclear HMGB1 release. A Pre-treatment of WT mice with Panx1 channel inhibitor TAT-Panx308 (i.p. administration 30 min prior to the start of optogenetic stimulation) prevented the elevation of MGS scores at 30 min following CSD, compared to mice pre-treated with scrambled (control) protein (TAT-Panxsc). B TAT-Panx308 pre-treatment prevented the increase of strokes at 30 min following CSD, compared to control TAT-Panxsc administration. C TAT-Panx308 pre-treatment prevented the increase nuclear HMGB1 release in both the ipsi- and contralateral to somatosensory cortex, compared to control TAT-Panxsc administration. D Representative photomicrographs of ipsilateral somatosensory cortex of WT mice 30 min after CSD, pretreated with TAT-Panx308 or TAT-Panxsc, stained for HMGB1 (red; nuclear Hoechst staining in blue). * p < 0.05. Scale bar: 250 μm