Table 1 Primer sequences used for plasmids’ cloning
SNP | Lenght (bp) | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
VAMP2_rs1150 | 1437 | CTGAACGATGGctgaaatctctggcctcacc | GTTGAAGGCTCTCgttcaagcaattctctgcct |
pGL3-promotor/VAMP2_rs1150 | 5028 | gaattgcttgaacGAGAGCCTTCAACCCAGTC | cagagatttcagCCATCGTTCAGATCCTTATCG |
SNAP25_rs2327264 | 1538 | GATCTGAACGATGGgcagttccctcactcatc | GTTGAAGGCTCTCgaatgccataatagcagctg |
pGL3-promotor/SNAP25_rs2327264 | 5028 | ctattatggcattcGAGAGCCTTCAACCCAGTC | gagggaactgcCCATCGTTCAGATCCTTATCG |