Fig. 4

Representative images and bar graphs illustrating the quantity of BDA-labelled nerve terminals and co-localized BDA and orexin cell bodies in the LH. A Example image of Hoechst 33342 stained cells; B) Example image of BDA-labelled terminals in the LH; C) Example image of orexin cell bodies in the LH; D) Example image of co-localized BDA-labelled terminals and orexin cell bodies; BDA (green) and orexin cell bodies (magenta) - Scale bars represent 100µm; E) Quantification of the total number of BDA-labelled terminals in sham and RmTBI animals; F) Quantification of the total number of co-localized BDA-labelled terminals and orexin cells in the LH of sham and RmTBI animals; G) Example image, with zoom (scale 10 µm) of co-localized BDA-labelled terminals and orexin cell bodies. Mean ± SEM shown