CGRP induces migraine-like symptoms in mice during both the active and inactive phases

Wattiez, Anne-Sophie; Gaul, Olivia J.; Kuburas, Adisa; Zorrilla, Erik; Waite, Jayme S.; Mason, Bianca N.; Castonguay, William C.; Wang, Mengya; Robertson, Bennett R.; Russo, Andrew F.

doi:10.1186/s10194-021-01277-9

The Journal of Headache and Pain

Table 1 Statistical analysis

From: CGRP induces migraine-like symptoms in mice during both the active and inactive phases

Figure #	Analysis		Statistics
Figure 1B Left panel	Two-way RM ANOVA	Interaction factor	F_(22,1276) = 0.759, p = 0.779
		Time factor	F_(11.8684.7) = 34.38, p < 0.0001
		Treatment factor	F_(1,58) = 1.718, p = 0.195
Figure 1B Right panel	Unpaired t-test		p = 0.0063
Figure 1C Left panel	Two-way RM ANOVA	Interaction factor	F_(22,1276) = 1.191, p = 0.245
		Time factor	F_{(10.2, 591.7)} = 35.78, p < 0.0001
		Treatment factor	F_(1,58) = 1.366, p = 0.247
Figure 1C Right panel	Unpaired t-test		p = 0.0035
Figure 1D Left panel	Two-way RM ANOVA	Interaction factor	F_(22,1276) = 1.317, p = 0.148
		Time factor	F_(9.22,531.1) = 54.93, p < 0.0001
		Treatment factor	F_(1,58) = 0.196, p = 0.660
Figure 1D Right panel	Unpaired t-test		p = 0.0042
Figure 2A 10 am (up^a)	Two-way RM ANOVA	Interaction factor	F_(23,299) = 1.012, p = 0.449
		Time factor	F_(1.04,13.58) = 73.07, p < 0.0001
		Treatment factor	F _[1_, 13_]=1.063, p = 0.321
Figure 2A 8 pm (up^a)	Two-way RM ANOVA	Interaction factor	F_(24,264) = 0.769, p = 0.779
		Time factor	F_(1.07,11.77) = 117.7, p < 0.0001
		Treatment factor	F _[1_, 11_]=0.757, p = 0.403
Figure 2B 8 pm (up^a)	Two-way RM ANOVA	Interaction factor	F_(48,600) = 3.025, p < 0.0001
		Time factor	F_(1.57,19.69) = 68.33, p < 0.0001
		Treatment factor	F _[1_, 13_]=7.246, p = 0.0185
Figure 2B 10 am (up)	Two-way RM ANOVA	Interaction factor	F_(48,574) = 8727, p = 0.715
		Time factor	F_(1.36,16.27) = 88.28, p < 0.0001
		Treatment factor	F _[1_, 13_]=1.335, p = 0.2687
Figure 2C	Unpaired t-test first hour		p = 0.0242
Figure 2C	Unpaired t-test second hour		p = 0.5009
Figure 2D	Unpaired t-test first hour		p = 0.0213
Figure 2D	Unpaired t-test second hour		p = 0.1474
Figure 3B Left panel	Baseline 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 2.813, p = 0.0209
		Time factor	F_{(3.435,61.83)} = 2.371, p = 0.071
		Treatment factor	F _[1_, 18_]=0.3919, p = 0.539
	Test 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 2.813, p = 0.0006
		Time factor	F_{(3.048,54.86)} = 2.228, p = 0.094
		Treatment factor	F _[1_, 18_]=14, p = 0.0015
	Test 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 1.048, p = 0.395
		Time factor	F_{(3.492,62.85)} = 8.281, p < 0.0001
		Treatment factor	F _[1_, 18_]=18.57, p = 0.0004
Figure 3B Right panel	Unpaired t-tests	Baseline 10 am	p = 0.539
		Test 10 am	p = 0.0015
		Test 8 pm	p = 0.0004
Figure 4B Left panel	Baseline 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 1.726, p = 0.137
		Time factor	F_(4.01,72,18) = 3579, p = 0.0101
		Treatment factor	F _[1_, 18_]=0.4585, p = 0.507
	Test 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 1.545, p = 0.184
		Time factor	F_{(3.882,69.88)} = 5.956, p = 0.0004
		Treatment factor	F _[1_, 18_]=19, p = 0.0004
	Test 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 0.5679, p = 0.724
		Time factor	F_{(2.612,27.02)} = 0.7879, p = 0.491
		Treatment factor	F _[1_, 18_]=6.589, p = 0.0194
Figure 4B Right panel	Unpaired t-tests	Baseline 8 pm	p = 0.507
		Test 8 pm	p = 0.0004
		Test 10 am	p = 0.0004
Figure 5A	Baseline 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 1.177, p = 0.327
		Time factor	F_{(3.586, 64.55)} = 25.17, p < 0.0001
		Treatment factor	F _[1_, 18_]=0.006, p = 0.936
	Test 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 3.653, p = 0.0047
		Time factor	F_{(3.714,66.84)} = 12.94, p < 0.0001
		Treatment factor	F _[1_, 18_]=27.78, p < 0.0001
	Test 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 0.4425, p = 0.818
		Time factor	F_(2.98,53.64) = 41.79, p < 0.0001
		Treatment factor	F _[1_, 18_]=26.79, p < 0.0001
Figure 5B	Baseline 10 AM Mixed-effects analysis
		Interaction factor	F_(5,87) = 0.4525, p = 0.810
		Time factor	F_{(2.659,46.26)} = 5.407, p = 0.0039
		Treatment factor	F _[1_, 18_]=0.025, p = 0.876
	Test 10 AM Mixed-effects analysis
		Interaction factor	F_(5,73) = 1.831, p = 0.117
		Time factor	F_{(3.419,49.92)} = 7.620, p = 0.0002
		Treatment factor	F _[1_, 18_]=1.271, p = 0.274
	Test 8 PM Mixed-effects analysis
		Interaction factor	F_(5,61) = 4.364, p = 0.0018
		Time factor	F_{(1.896,24.22)} = 8.867, p = 0.0013
		Treatment factor	F _[1_, 18_]=0.0172, p = 0.897
Figure 5C	Baseline 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 0.9072, p = 0.907
		Time factor	F_{(3.335,60.04)} = 29.30, p < 0.0001
		Treatment factor	F _[1_, 18_]=0.0558, p = 0.8159
	Test 8 PM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 0.86, p = 0.511
		Time factor	F_{(3.635,65.44)} = 32.49, p < 0.0001
		Treatment factor	F _[1_, 18_]=7.239, p = 0.0150
	Test 10 AM Two-way RM ANOVA
		Interaction factor	F_(5,90) = 1.349, p = 0.2510
		Time factor	F_{(3.005,54.08)} = 18.67, p < 0.0001
		Treatment factor	F _[1_, 18_]=3.494, p = 0.0779
Figure 5D	Baseline 8 PM Mixed-effects analysis
		Interaction factor	F_(5,86) = 1.114, p = 0.359
		Time factor	F_{(2.837,48.79)} = 12.33, p < 0.0001
		Treatment factor	F _[1_, 18_]=0.2415, p = 0.629
	Test 8 PM Mixed-effects analysis
		Interaction factor	F_(5,77) = 1.786, p = 0.126
		Time factor	F_{(3.619,55.74)} = 7.105, p = 0.0002
		Treatment factor	F _[1_, 18_]=0.1405, p = 0.712
	Test 10 AM Mixed-effects analysis
		Interaction factor	F_(5,82) = 1.142, p = 0.3449
		Time factor	F_{(32.051,33.63)} = 5.67, p = 0.0071
		Treatment factor	F _[1_, 18_]=0.1632, p = 0.6910
Figure 6B	Two-way RM ANOVA
		Interaction factor	F _[1_, 25_]=20.95, p < 0.0001
		Time factor	F _[1_, 25_]=4.953, p = 0.0034
		Treatment factor	F _[1_, 25_]=7.707, p = 0.0095
Figure 6D	Two-way RM ANOVA
		Interaction factor	F _[1_, 26_]=13.66, p = 0.0009
		Time factor	F _[1_, 26_]=7.585, p = 0.0099
		Treatment factor	F _[1_, 26_]=9.298, p = 0.0008

RM Repeated measures; up^a: underpowered separate when plotted alone; multiple comparison analysis detailed in figure legends when needed

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ISSN: 1129-2377

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