Unilateral facial injection of Botulinum neurotoxin A attenuates bilateral trigeminal neuropathic pain and anxiety-like behaviors through inhibition of TLR2-mediated neuroinflammation in mice

Table 2 The primers used in RT-qPCR

	Forward primers	Reverse primers	Accession No.
Gapdh	TTGATGGCAACAATCTCCAC	CGTCCCGTAGACAAAATGGT	NM_001001303
Tlr1	ATGTGAGCTGAGGGACTTTG	GGATAGTGGAGACATGTGGAAG	NM_030682
Tlr2	ACCAAGATCCAGAAGAGCCA	CATCACCGGTCAGAAAACAA	NM_011905
Tlr3	GCGTTGCGAAGTGAAGAACT	TTCAAGAGGAGGGCGAATAA	NM_126166
Tlr4	TTCAGAACTTCAGTGGCTGG	TGTTAGTCCAGAGAAACTTCCTG	NM_021297
Tlr5	GCAGGATCATGGCATGTCAAC	ATCTGGGTGAGGTTACAGCCT	NM_016928
Tlr6	CCAAGAACAAAAGCCCTGAG	TGTTTTGCAACCGATTGTGT	NM_011604
Tlr7	GATGTCCTTGGCTCCCTTC	TTTGTCTCTTCCGTGTCCAC	NM_133211
Tlr8	CGTTTTACCTTCCTTTGTCTATAGAAC	CGTCACAAGGATAGCTTCTGG	NM_133212
Tlr9	AACCGCCACTTCTATAACCAG	GTAAGACAGAGCAAGGCAGG	NM_031178
Tlr11	CAGGCTGGGATTGCTCATC	CCAGTCAAGGTAAGGCTCAC	NM_205819
Tlr12	GCTCTGATTCCTCTGGTGTAG	AGAATGTGAAATAGCGGGAGAC	NM_205823
Tlr13	GGAGCGCCTTGATCTAACTAACA	TCAGGTGGGTCAGAGAAACCA	NM_205820
IL-1β	AGAGCATCCAGCTTCAAATCTC	CAGTTGTCTAATGGGAACGTCA	NM_008361.4
TNF-α	AGCCGATGGGTTGTACCTTG	TTGGGCAGATTGACCTCAGC	NM_001278601.1
IL-6	TCAGGAAATTTGCCTATTGAAAA	CCAGCTTATCTGTTAGGAGAGCA	NM_031168.2
c-Fos	GACAGCCTTTCCTACTACCA	CATCTTATTCCTTTCCCTTCG	XM_021201871.2
F4/80	ATGGACAAACCAACTTTCAAGGC	GCAGACTGAGTTAGGACCACAA	XM_036160297.1
GFAP	GAATCGCTGGAGGAGGAGAT	GCCACTGCCTCGTATTGAGT	XM_030245571.2
CD11b	GACATGGACGCTGATGGCAATAC	CGAGGCAAGGGACACACTGAC	NM_008401.2

ISSN: 1129-2377