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Fig. 4 | The Journal of Headache and Pain

Fig. 4

From: Unilateral facial injection of Botulinum neurotoxin A attenuates bilateral trigeminal neuropathic pain and anxiety-like behaviors through inhibition of TLR2-mediated neuroinflammation in mice

Fig. 4

Expression of TLRs in bilateral TNC using RT-qPCR. a RT-qPCR analyzed the expression levels of TLRs in wild-type mice. GAPDH was used as internal control (n = 6). b Relative expression of TLRs in the ipsilateral TNC on day 5 after BoNT/A injection (day 19 following dIoN-CCI surgery) among the three groups. There was an increase in TLR2 and TLR5, a decrease in TLR11 in the dIoN-CCI group compared with the sham group, while there was a decrease in TLR1, TLR2, TLR4, TLR5 and TLR8 expression after BoNT/A injection. c Relative expression of TLRs in the contralateral TNC on day 5 after BoNT/A injection (day 19 following dIoN-CCI surgery) among the three groups. The dIoN-CCI and sham group showed no significant change in TLRs expression, while TLR1, TLR4 and TLR8 expression decreased after BoNT/A injection. Data was expressed as Mean ± SEM. (n = 4 each group, *** P < 0.001 compared with the sham group. # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the dIoN-CCI group, unpaired Student’s t-test). TLRs, toll-like receptors; TNC, trigeminal nucleus caudalis; dIoN-CCI, chronic constriction injury of distal infraorbital nerve; BoNT/A, botulinum toxin–A; BL, baseline

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The Journal of Headache and Pain

ISSN: 1129-2377

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