Plasma levels of CGRP and expression of specific microRNAs in blood cells of episodic and chronic migraine subjects: towards the identification of a panel of peripheral biomarkers of migraine?

Table 4 Comparison of biochemical variables between EM and CM-MO patients after detoxification

	EM	CM-MO at T1			Kruskal-Wallis test	Post-hoc
	EM	All patients	Responders	Non-responders	Kruskal-Wallis test	Responders vs. EM	Responders vs. non-responders	Non-responders vs. EM
n	27	28	23	5		–
CGRP (pg/ml)	220.4 ± 84.4	275.2 ± 251.8	251.8 ± 383.2	383.2 ± 208.7	0.472	–	–	–
miR-382-5p (RQ)	3.3 ± 0.63	2.2 ± 0.4	1.7 ± 0.3	4.4 ± 1.1	0.006	0.042	0.034	1.000
miR-34a-5p (RQ)	18.4 ± 4.7	21.6 ± 4.0	20.7 ± 4.4	25.9 ± 10.4	0.248	–	–	–

Legend: EM episodic migraine, CM-MO chronic migraine with medication overuse. Responders: patients with a reduction of at least 30% in monthly migraine days after detoxification. CGRP values are presented as mean ± standard deviation. microRNAs values are presented as mean ± standard error of the mean. RQ Relative quantification: 2^−∆∆Ct = 2 − ^{(∆Ct gene − ∆Ct housekeeping gene)}, Ct cycle threshold as defined in methods section. Statistical analysis: differences among EM groups, responders, and non-responders patients were analyzed with Kruskal-Wallis test followed by a post-hoc analysis; the levels of significance were further corrected with a Bonferroni method to account for multiple comparison

ISSN: 1129-2377